Abstract
Objective To observe the effects of heat-CO2-treated dendritic cells (DC)-derived dexosomes (DEXs) on apoptosis of human gastric adenocarcinoma cell line AGS and investigate the applied value of heat-CO2 pneumoperitoneum during laparoscopic surgery.Methods An in vitro heat-CO2 pneumoperitoneum experimental box was built.After heat-CO2 pneumoperitoneum treatment,the DC-derived DEXs were isolated and identified through TEM and Western blotting.AGS cells were co-cultured with DEXs,apoptosis was examined by Annexin V/propidium iodide (PI) flow cytometry and Hoechst 33258 fluorescent microscopy,and relative Caspase-3 activity was determined by using colorimetric assay.Results DEXs were successfully isolated.Heat-CO2-treated DC-derived DEXs produced evident anti-proliferative effect and the cell proliferation inhibition rate at 1st,2nd,3rd and 4th day was 31.13％,43.28％,51.34％ and 50.32％ respectively.The heat-CO2-treated CD-derived DEXs induced apoptosis of AGS cells.The early apoptosis rate induced by DEXs was 2.3％,5.6％,15.7％ and 36.8％ respectively.The relative viability of AGS cells in heat-CO2-treated DC-derived DEXs group was 171.37％.Conclusion Heat-CO2 treated DC-derived DEXs could significantly inhibit proliferation and induce apoptosis of AGS cells,which may be related to the activation of Caspase-3. Key words: Heat-CO2 ; Dendritic cell; Gastric adenocarcinoma; Apotosis
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