Devosia nitraria sp. nov., a novel species isolated from the roots of Nitraria sibirica in China

Lin Xu,Yong Zhang,Nicholas Read,Shuangjiang Liu,Ville-Petri Friman

doi:10.1007/s10482-017-0901-z

Abstract

An aerobic, Gram-stain negative, short rod-shaped and motile strain, 36-5-1T, was isolated from the roots of Nitraria sibirica in Zhangye city, Gansu province, north-west of China. Phylogenetic analysis based on the 16S rRNA gene sequence and two housekeeping genes (glnA and atpD) indicated that the strain represents a novel species closely related to the Devosia, Rhizobium and Devosia genera with 98.3, 96.2 and 91.1% similarities, respectively. The strain 36-5-1T contained Q-10 as the predominant ubiquinone and 16:0 (36.8%) as the major fatty acid; a large amount of unidentified glycolipid, diphosphatidylglycerol, phosphatidylglycerol and a small amount of unidentified polar lipids were present as polar lipids. In addition, the G+C content of the genomic DNA was 61.7 mol% and the DNA–DNA hybridization with type strains Devosia geojensis BD-c194T and Devosia pacifica NH131T 44.1 ± 1.1 and 40.2 ± 1.7, respectively. Based on chemotaxonomic data and molecular properties, strain 36-5-1T represents a novel species within the genus Devosia, for which the name Devosia nitraria sp. nov. is proposed. The type strain is 36-5-1T (=CGMCC1.15704T=NBRC112416T).

Highlights

Based on chemotaxonomic data and molecular properties, strain 36-5-1T represents a novel species within the genus Devosia, for which the name Devosia nitraria sp. nov. is proposed
The genus Devosia was first described by Nakagawa et al (1996) with the reclassification of ‘Pseudomonas riboflavina’ (Foster 1944) as Devosia riboflavina
The phylogenetic trees based on 16S RNA genes indicated that the strain clustered with species of the genus Devosia

Summary

Introduction

The genus Devosia was first described by Nakagawa et al (1996) with the reclassification of ‘Pseudomonas riboflavina’ (Foster 1944) as Devosia riboflavina. The G?C content of the genomic DNA was 61.7 mol% and the DNA–DNA hybridization with type strains Devosia geojensis BD-c194T and Devosia pacifica NH131T 44.1 ± 1.1 and 40.2 ± 1.7, respectively. Based on chemotaxonomic data and molecular properties, strain 36-5-1T represents a novel species within the genus Devosia, for which the name Devosia nitraria sp. Keywords Devosia Á Novel species Á Nitraria sibirica

Results

Conclusion