Devosia geojensis sp. nov., isolated from diesel-contaminated soil in Korea

Abstract

A Gram-negative, strictly aerobic, non-spore-forming bacterium, designated strain BD-c194(T), was isolated from diesel-contaminated soil in Geoje, Korea. The cells were short, motile rods with single polar flagella. Growth of strain BD-c194(T) was observed between 15 and 45 degrees C (optimally at 30-35 degrees C) and between pH 6.0 and 9.5 (optimally at pH 7.5-9.0). The predominant fatty acids were 11-methyl C(18 : 1)omega7c, C(16 : 0), C(18 : 1)omega7c, C(18 : 0) and an unknown fatty acid (equivalent chain-length 18.814); a large amount of phosphatidylglycerol and a small amount of diphosphatidylglycerol were present as polar lipids. The G+C content of the genomic DNA was 60.8 mol% and the major isoprenoid quinone was Q-10. A comparative 16S rRNA gene sequence analysis showed that strain BD-c194(T) formed a well-defined phyletic lineage within the genus Devosia (with 100 % bootstrap support). The levels of 16S rRNA gene sequence similarity with respect to the type strains of other Devosia species ranged from 95.0 to 96.1 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c194(T) represents a novel species within the genus Devosia, for which the name Devosia geojensis sp. nov. is proposed. The type strain is BD-c194(T) (=KCTC 22082(T) =DSM 19414(T)).