Developmental Trenbolone Exposure Affects Adult Breeding Behavior, Fecundity and Morphology ofXenopus tropicalis

Abstract

Event Abstract Back to Event Developmental Trenbolone Exposure Affects Adult Breeding Behavior, Fecundity and Morphology of Xenopus tropicalis Robert A. Miranda1*, Allen W. Olmstead2, Lindsey S. Blake2, Sigmund J. Degitz2, David R. Garcia1, Atilade A. Owolabi1 and Catherine R. Propper1 1 Northern Arizona University, Biological Sciences, United States 2 US Environmental Protection Agency, Mid-Continent Ecology Division, United States Trenbolone acetate is a synthetic androgen used as a growth promoter in the cattle industry. Its metabolite 17β-trenbolone (17β-T) has been detected downstream from cattle feedlots. It could be a concern to wildlife near these areas as previous studies show 17β-T exposure affects reproductive endpoints in fishes. This study evaluated the impact of developmental aqueous exposure to17β-T on adult breeding behavior, fecundity and morphology in the Western clawed frog, Xenopus tropicalis. Tadpoles were exposed to 17β-T (0, 3.7, 11, 33 or 100 ng/L) in 4 L of water using a flow-through system until metamorphosis then raised to adulthood in clean water. Breeding trials, using pairs within exposure levels (n=10; n=9 for 100 ng/L), were conducted to evaluate the number and viability of eggs released. None of the females in the 100 ng/L treatment and only one female from the 33 ng/L treatment released eggs. There was no difference in the number of eggs released and the proportion of eggs that were viable among the control and two lower exposure treatments. Breeding behaviors from the trials were also filmed and evaluated. During the first three hours of behavior observation, there was no difference in the number of male clasp attempts (Kruskal-Wallis: p=0.13), but there was a decrease in time frogs spent in amplexus for the highest treatment (Kruskal-Wallis: p=0.037). Frogs were then euthanized and tissues were collected to evaluate morphological endpoints and brain gene expression (not reported here). Males and females did not differ in body size, or mass of body mass specific liver, kidneys, gonads or fat bodies. Females exposed to100 ng/L 17β-T did not develop oviducts, and only two females exposed to 33 ng/L 17β-T had completely developed oviducts. Our results demonstrate that developmental exposure to 17β-T impacts reproduction in an aquatic vertebrate by acting at multiple endpoints. Keywords: Xenopus tropicalis, Behavior, endocrine disruption, fecundity, trenbolone Conference: ISAREN 2011: 7th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology, Ann Arbor, United States, 11 Jul - 13 Jul, 2011. Presentation Type: Oral Presentation Topic: Endocrine disruption Citation: Miranda RA, Olmstead AW, Blake LS, Degitz SJ, Garcia DR, Owolabi AA and Propper CR (2011). Developmental Trenbolone Exposure Affects Adult Breeding Behavior, Fecundity and Morphology of Xenopus tropicalis. Front. Endocrinol. Conference Abstract: ISAREN 2011: 7th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology. doi: 10.3389/conf.fendo.2011.03.00032 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Jul 2011; Published Online: 09 Aug 2011. * Correspondence: Mr. Robert A Miranda, Northern Arizona University, Biological Sciences, Flagstaff, AZ, United States, ram257@nau.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Robert A Miranda Allen W Olmstead Lindsey S Blake Sigmund J Degitz David R Garcia Atilade A Owolabi Catherine R Propper Google Robert A Miranda Allen W Olmstead Lindsey S Blake Sigmund J Degitz David R Garcia Atilade A Owolabi Catherine R Propper Google Scholar Robert A Miranda Allen W Olmstead Lindsey S Blake Sigmund J Degitz David R Garcia Atilade A Owolabi Catherine R Propper PubMed Robert A Miranda Allen W Olmstead Lindsey S Blake Sigmund J Degitz David R Garcia Atilade A Owolabi Catherine R Propper Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.