Developmental stability in flowers of Clarkia tembloriensis (Onagraceae)

Abstract

AbstractDevelopmental instability of floral traits is examined in four populations of Clarkia tembloriensis (Onagraceae) with different natural outcrossing rates. Developmental instability is estimated using fluctuating asymmetry (FA) and within plant variance. The results are coupled with those from a previous study of leaf traits.In the first experiment, flowers were collected from the same growth chamber‐grown plants that had been previously used to estimate leaf developmental stability in two C. tembloriensis populations. These populations differed in FA for only one floral trait, long filament length. After adjusting for organ size differences, we found floral FA values were about half those of leaves. These are the first quantitative data indicating that flowers are more developmentally stable than leaves.In a second experiment, greenhouse grown plants from two other C. tembloriensis populations (one highly outcrossing and one predominantly self‐pollinating) did not differ significantly in floral FA or in within‐plant variance of floral traits, though earlier studies of the same populations revealed significant differences in FA of leaf traits.In both experiments, FA values of different floral traits were uncorrelated.We attribute the lack of significant differences in floral stability between populations to the greater canalization of floral organs and to the magnification of measurement error that occurs when calculating FA. We also found that the shorter styles of selfers are the greatest difference in flower form between predominantly self‐pollinating and predominantly outcrossing populations of C. tembloriensis.