Abstract
The Adh1 gene coding for alcohol dehydrogenase (ADH) enzyme is one of the most widely studied genes in plants. Some controversy exists, however, concerning the induction of ADH activity in soybean [Glycine max (L.) Merr.] by flooding stress. Thistudy showed that the induction of ADH in 5‐ to 21‐d‐old soybean seedlings by flooding was organ‐specific and changed with seedling age. A single ADH isozyme was resolved by native polyacrylamide gel electro‐phoresis of protein extracts from control and flooded soybean leaves, hypocotyls, and roots. The cotyledons of unstressed soybean contained three ADH isozymes: two were distinct, one was faintly visible. Following plant submersion, all three isozymes in the cotyledons increased in activity. RNA blot hybridization revealed the accumulation of Adh‐specific transcripts in flooded soybean cotyledons and roots. A two‐gene model for soybean ADH is proposed, where one gene is expressed in roots, hypocotyls, leave, and cotyledons, while the other gene is expressed only in cotyledons.
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