Abstract
Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P
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