Abstract
Using both concanavalin A crossed-line affinity immunoelectrophoresis and lentil lectin crossed-line affinity immunoelectrophoresis, developmental changes of alpha-fetoprotein (AFP) subfractions were studied in 11 samples of human amniotic fluid obtained between 43 and 121 days of gestation. In the amniotic fluid at 43 or 48 days of gestation, only subfractions produced by the yolk sac were evident. The initial appearance of the subfractions produced by the fetal liver was first detected at 51 days of gestation. Percentages of liver-originated subfractions rose rapidly with gestational age and reached a level of 50% at 54 days of gestation. The subfractions produced by the yolk sac disappeared from the amniotic fluid at 121 days of gestation. These findings suggest that, in early gestation, the yolk sac is mainly responsible for the fetal AFP synthesis and that initiation of AFP production by the fetal liver takes place between 48 and 51 days of gestation. In the present study, attention was also given to the origin of four subfractions separated by the lentil lectin crossed-line affinity immunoelectrophoresis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.