Developmental changes in the response of larval Manduca sexta fat body glycogen phosphorylase to starvation, stress and octopamine

Abstract

Fasting or starvation of 1 st- and 2 nd-day fifth instar Manduca sexta larvae leads to rapid activation of fat body glycogen phosphorylase. Under feeding conditions, 21–29% of the phosphorylase was found in the active form. However, after only one hour of starvation, the active form increased to 55–65%. In larvae on the 3 rd-day there was a slower increase in the activation, requiring three hours of starvation to reach a maximum of 60–65%. No activation was observed in 4 th-day larvae after three hours of starvation. When 1 st- or 2 nd-day larvae were decapitated, the time-course of activation of glycogen phosphorylase was very similar to that observed in intact insects. However, activation of glycogen phosphorylase following decapitation was only observed in 1 st- and 2 nd-day larvae. In 2 nd-day larvae, octopamine promoted activation of glycogen phosphorylase and 100-pmol of octopamine promoted maximum activation. Higher amounts of injected octopamine caused a decrease in activation. The injection of 100 pmol of octopamine caused a 50–55% activation of phosphorylase within 30 minutes. The simultaneous injection of the α-adrenergic receptor antagonist phentolamine with octopamine blocked the octopamine effect in 1 st- and 2 nd-day feeding larvae. However, the activation of glycogen phosphorylase observed in ligated/decapitated larvae on the 1 st- and 2 nd-day was not abolished by injection of phentolamine. All of these data suggest that factors other than adipokinetic hormone and octopamine may be involved in the activation of glycogen phosphorylase during fasting or starvation in the early part of the fifth larval stage of M. sexta.