Developmental changes in the expression of Shaker- and Shab-related K + channels in neurons of the rat trigeminal ganglion

Abstract

We have investigated properties of voltage-gated K + channels in neurons of the pre- and postnatal rat trigeminal ganglion (TG). To correlate functional data with information on gene expression of Shaker- and Shab-related channels in these pseudo-unipolar neurons, the patch-clamp technique was combined with the single-cell reverse transcription-polymerase chain reaction (RT-PCR). A majority (80%) of prenatal TG neurons possessed only sustained delayed rectifier currents with half-maximal current inactivation at −30 mV. In the postnatal cells, steady-state inactivation of sustained currents occurred at more negative voltages (half-maximal inactivation at −58 mV). About 65% of the postnatal cells displayed a transient outward component in addition to the sustained currents. With increasing age, the sensitivity of sustained currents to 4-aminopyridine (4-AP) decreased significantly. The Shaker channel toxins, α-dendrotoxin and agitoxin-2 (50 and 10 nM), were much less effective. Discrimination between both stages with tetraethylammonium chloride (5 mM) was not possible since the currents were reduced generally by about 50%. After recording, the cell content was harvested and single-cell RT-PCR was performed to compare K + current properties and mRNA expression within the same cell. Most cells simultaneously expressed several different Shaker- and Shab-like transcripts. At postnatal day 14, the frequency of cells carrying transcripts encoding Kv1.1 decreased. Detailed analysis revealed a higher 4-AP sensitivity of TG neurons expressing Kv1.1 transcripts.