Developmental changes in the expression of α-, β- and γ-subspecies of protein kinase C at synapses in the ventral horn of the embryonic and postnatal rat spinal cord

Abstract

Developmental changes in expression of α-, β- and γ-subspecies of protein kinase C (PKC) at synapses in the ventral horn of the rat spinal cord were immunocytochemically investigated. On embryonic day 15, a few synapses were found in the ventral horn, and they gradually ncreased in number until postnatal day 21 or 28. During the embryonic period, immunoreactivity (IR) for all three subspecies was demonstrated in both the pre- and postsynaptic regions. In the former, IR was detected mainly along the outer surface of the synaptic vesicles, and in the latter, along the postsynaptic membranes. At these stages, synapses were morphologically immature, having a faint postsynaptic density and a few round synaptic vesicles. After birth, IR for PKCs at the postsynaptic densities became stronger, but gradually disappeared in most of the presynaptic regions. In adult, IR for PKCs was detected only at the postsynaptic densities. At the later postnatal stages, the synapses were fully mature, having a thick postsynaptic density, a great number of synaptic vesicles and a distinct synaptic cleft as those in adult animals. In addition, the developmental changes in expression of these subspecies of PKC in the presynaptic regions were quite different. These findings suggest that the increase in expression of PKC at postsynaptic densities might be closely related with the development of synaptic functions, and also that each subspecies of PKC may take part in different aspects of synaptogenesis.