Abstract
1. At time zero, scattered basophilic granules (BG) were observed between the vitelline and the ectoblast (ectoderm). In addition, there were BGs in the spaces between the cells of the germ layer. 2. Five hours incubated egg: BGs were sometimes scattered, congregated (grouped), or arranged in order. 3. Seven to eight hours: Mesoderm (mesoblast) began to differentiate. Special looking cells which were different from mesoblast cells appeared between the ectoderm and mesoderm in the area pellucida (bright area) and the area opaca (dark area). These cells were of long oval shape. Short protuberances sprouted from the long axis, and the cell appeared to be a bipolar cell.
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