Developmental analysis of the peripheral olfactory organ of the opossum Monodelphis domestica

Abstract

The gray, short-tailed opossum, Monodelphis domestica, is born in a very immature state after a brief (14-day) gestation. As a result, the species provides a unique opportunity to examine very early periods of mammalian development. The present study provides the first detailed morphometric analysis of the development of the olfactory mucosa and the nasal cavity in Monodelphis. The extent of the sensory mucosa increases dramatically across development, covering a growing nasal cavity and increasingly elaborate turbinates. Both nasal cavity convolution (a measure of turbinate complexity) and mucosal surface area show extensive growth between birth and adulthood. These measurements are greatest in the central portion of the mucosa (in the caudal portion of the nose) at all ages examined. A developmental BrdU study reveals a robust decrease in cellular proliferation with age; proliferation decreases to near adult-like patterns by postnatal day (P) 40. Results from these studies show that there is dramatic structural and cellular postnatal growth in the opossum peripheral olfactory organ.