Development Research of an ADT-24 Multichannel Real-time Fluorescence PCR Detection System

Abstract

This study describes a fluorescence detection system for purified ADT-24 nucleic acids and a PCR detection-integrated machine. The application background and structure of the fluorescence scanning system are described. We designed a confocal 4-channel fluorescence detection scanning head with monochromatic LEDs as the excitation light source, a multipixel photon counter (MPPC) as the optical path detection terminal, and a narrow-band filter and dichroic mirror as the main optical components. This paper discusses in detail the principle of the working system, including optical path selection and standards, optical component selection, channel spectral characteristic analysis, detector selection, performance evaluation, optical path simulation, and sensitivity verification. The design and use of this optical system are also summarized. Finally, a 4-fold dilution of mix reagent was used to test the detection effect of the fluorescence scanning head, and a scheme to control “temperature drift” and optimize performance was proposed. According to the verification results and subsequent live debugging, the debugged results achieved the expected design objectives.