Abstract
In this report, a rapid and selective analytical method for the determination of monoterpenes in Alpinia zerumbet essential oil (AZEO) by gas chromatography (GC) flame ionization detection (FID) was developed, optimized and validated. The suitability of six different capillary columns was investigated by polarity phase constants calculated based on the methodology proposed by Rohrschneider and McReynolds. The most suitable column was then used in a central composite design applying the following GC factors to investigate responses based on peak resolutions and analysis time: initial oven temperature, heating rate and carrier gas flow rate. The optimized method has initial oven temperature of 60 oC, heating rate of 5.1 oC min-1 and flow rate of 1.0 mL min-1 using the DB-35 capillary column. The validation acceptance criteria were met in all cases. The method was successfully applied for the quantification of major monoterpenes found in AZEO in different samples of Alpinia zerumbet.
Highlights
The Alpinia genus includes a various number of aromatic species that have extensive medicinal uses in various parts of Asia and the Americas
The identity obtained from mass spectral analysis and the calculated linear retention indices (LRI) of each AZ1 essential oil constituent are summarized in Table 3, and the components are reported according to the chromatography elution order in DB-5MS column
The gas chromatography (GC)-mass spectrometry (MS) preliminary analysis of the essential oil provided the identification of 21 compounds using their LRI, their mass spectra and/or by co-injection with their authentic reference standards
Summary
The Alpinia genus includes a various number of aromatic species that have extensive medicinal uses in various parts of Asia and the Americas. The medicinal properties of this species are related to different parts of the plant, including leaves, flowers and rhizomes.[2] A field research conducted in Ibiúna, São Paulo, Brazil, revealed the use of A. zerumbet volatile fraction in respiratory disorders.[3] In the state of Pará, Brazil, “colônia” flowers tea was widely used as sedative,[4] for chest pain and headache.[5] A. zerumbet is mostly recognized for its use as anti-hypertensive, normally using infusions or decoctions of its leaves as traditional medicine.[6,7]
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