Development of wound healing spray from keratin protein

Mohamed Saad Bala Mohamed Saad Bala,Basma Yahya Alashwal Basma Yahya Alashwal,Arun Gupta Arun Gupta,Kan Weng Chin Kan Weng Chin

doi:10.54279/mijeec.v2i2.245032

Mohamed Saad Bala Mohamed Saad Bala, Basma Yahya Alashwal Basma Yahya Alashwal + Show 2 more

Open Access

https://doi.org/10.54279/mijeec.v2i2.245032

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Abstract

Wound healing spray was developed from keratin protein extracted from a chicken feather in the study. Keratin is the most abundant protein in epithelial cells. Keratin was proven for wound healing ability because it can activate the keratinocyte in the skin responsible for wound healing. However, keratin wound healing spray is new to the market and has the potential to heal the wound gentle and pain-free. This study synthesized the keratin wound healing spray from chicken feathers and determined the character of the keratin wound healing spray. Keratin protein was extracted from chicken feathers. The extracted keratin solution was concentrated to the desired concentration by a rotary evaporator. The wound healing spray was synthesized by mixing the desired antimicrobial agent with keratin solutions like manuka honey, Garcinia Mangostana L., caprylyl glycol, ethylhexylglycerin and propylene glycol at a concentration of 13.0 w/w%, 0.5 w/w%, 1.0 w/w%, 2.0 w/w% and 2.0 w/w%, respectively, and the characteristic was determined. The result has shown that the keratin protein is maintained in the wound healing spray after mixing with an antimicrobial agent according to the formulation from the FTIR result. The wound healing spray does not contain heavy metals like cadmium and lead. Still, copper, iron and zinc were present within the maximum daily level of vitamins and minerals for adults allowed in health supplements by the National Pharmaceutical Regulatory Division of Malaysia. The pH of the keratin wound healing spray was maintained at a pH of around 5.5. The density and the viscosity of the keratin wound healing spray were higher than the deionized water. In conclusion, the Keratin wound healing spray was synthesized, and it is safe for the consumer. The wound healing ability of the keratin wound healing spray needs to carry out the in vivo clinical test for future study.

Highlights

Keratin is a group of filament-forming proteins with high sulphur content and insoluble
The keratin wound healing spray was suggested to be shaken before applying it to the wound
The keratin wound healing spray developed from the study contain the keratin protein after mixing with antimicrobial agents such as manuka honey, Garcinia Mangostana L., caprylyl glycol, ethylhexylglycerine, and propylene glycol, according to the Fourier-Transform Infrared Spectroscopy (FTIR) result

Summary

Introduction

Keratin is a group of filament-forming proteins with high sulphur content and insoluble. It was usually constituting in the bulk of epidermal such as claw, turtle shell and feather. It is the most abundant protein in epithelial cells because it's the largest subgroup of intermediate filament proteins that protect epithelial cells from nonmechanical and mechanical stress, which is the primary function of keratins (Coulombe & Omary, 2002; Husain et al, 2018; Abayomi et al, 2016). Keratin extracted from ovine wool can treat an acute wound, chronic wound and some skin disorders, epidermolysis bullosa (Kelly, 2016; Alashwal et al, 2020). Patients reported a faster healing rate and more resilient healed skin for Epidermolysis bullosa, a genetically inherited condition that will cause skin fragility and minor trauma leading to skin loss, damage, and wounding (Enter et al, 2015; Yu et al, 2017)

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Results

Conclusion