Development of validated UV spectrophotometric method for the simultaneous estimation of metformin hydrochloride and glipizide in tablet dosage form

Abstract

A simple precise reproducible U.V. Spectrophotometric method have been developed and validated for the simultaneous estimation of MET and GPZ in tablet dosage form. This paper describes the simultaneous equation method as a quantification parameter which involves the measurement of absorbance of MET and GPZ at 237 nm and 234.0 nm respectively. MET and GPZ both obeyed linearity in the range of 2?g/mL to 20 ?g/mL. The recovery studies shows %RSD for MET 0.005, 0.12, 0.65 and for GPZ 0.008, 0.54, 0.25 by SEM method. The results of analysis have been validated statistically for accuracy, Precision, Repeatability, and Ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage form