Abstract
Ethacridine lactate (EAL) is an antiseptic in solutions of 0.1%. It is also used as an agent for second trimester abortion. Two simple and sensitive spectrophotometric methods (Method A and Method B) were developed for the estimation of EAL in pharmaceutical formulations. Method A is based on the condensation of the primary aromatic amino group of ethacridine lactate with an aromatic aldehyde (p-Dimethylamino- cinnamaldehyde) to form a chromophore with absorption maximum of 600nm. Method B is based on redox reaction followed by complex formation of EAL with IO3-Metol reagent to form a stable chromogen, which can be estimated at 520 nm. Method A obeys Beer’s law in the concentration range of 2 to 12 μg/mL and method B in the range of 20 to 100 μg/mL. Interference studies were conducted to see the influence of excipients with the proposed methods. The common excipients usually present in dosage forms do not interfere in the proposed method A and method B. The optical characteristics, regression analysis data and precision of the methods were also calculated. The methods were validated for use in routine quality control of EAL in pharmaceutical formulations.
Highlights
Ethacridine lactate[1,2,3,4] is an antiseptic in solutions of 0.1%; it is used as an agent for second trimester abortion
The present investigation is to develop two simple and sensitive spectrophotometric methods for the estimation of Ethacridine lactate (EAL) in pharmaceutical formulations based on; (a) The condensation of the primary aromatic amino group of EAL with p-dimethylaminocinnamaldehyde (PDAC)[9] and (b) The redox reaction followed by complex formation of EAL with IO3-/metol reagent[10,11,12]
To a series of 10 mL volumetric flasks, methanolic EAL standard solution (100 μg/mL) ranging from 0.2 to 1.2 mL was transferred and 1.0 mL of PDAC followed by 1 mL of 10% sulfuric acid
Summary
Ethacridine lactate[1,2,3,4] is an antiseptic in solutions of 0.1%; it is used as an agent for second trimester abortion. PDAC solution (Loba, 0.2% w/v, 1.14x10-2 M): Prepared by dissolving 200 mg of p-dimethylaminocinnamaldehyde in 100 mL of methanol. KIO3 solution (Merck, 0.43%, 2.0x10-2 M): Prepared by dissolving 430 mg of potassium iodate in 100 mL of distilled water. The stock solution (1 mg/mL) of EAL was prepared by dissolving 100 mg of the EAL in 100 mL of methanol. The content of ten vials was taken and thoroughly mixed From this an accurately measured portion of the liquid content equivalent to 100 mg of the drug was taken and diluted to 100 mL with methanol. Later this solution was further diluted to get absorbance values within the calibration curve range
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