Abstract
We have to developed active microbial consortium that could be of higher degradation of crude oil contaminated groundwater, wastewater aeration pond and biopond at the oil refinery Terengganu Malaysia. Among four isolates that showed good growth only three different isolates (Acinetobacter faecalis WD2, Staphylococcus. sp DD3 and Neisseria elongate TDA4.) were selected based on the growth ability and degradation. Significant growth and effectiveness of hydrocarbon biodegradation of the bacterial consortium examined bacterial strains and their mixtures in both were observed after 5, 10 and 15 days of degradation. Gas chromatography showed that more than 96 and 98% degradation of total hydrocarbon by consortia sp respectively.
Highlights
Chemical treatment includes direct injection of chemical oxidants into contaminated soil and groundwater thereby altering native aquatic chemistry and biology
Media and culture condition: Bacterial strains were isolated from samples collected from groundwater and wastewater aeration pond and biopond located at the Terengganu oil refinery, Malaysia. 10ml of each sample were washed with 90mL saline and filtered with membrane
Extraction of residual crude oil for Gas Chromatography (GC) analysis: Crude oil was extracted with a pre-cleaned separating funnel, following the modified methods of[7]. 50 mL of sample (BM + crude oil + bacteria), were centrifuged at 4000 rpm for 15 min
Summary
Chemical treatment includes direct injection of chemical oxidants into contaminated soil and groundwater thereby altering native aquatic chemistry and biology. When the other 3 strains were removed from the consortium, the effectiveness of the mixed culture was remarkably reduced. These further support the theory that each member in a microbial community has a significant role and may need to depend on the presence of other species or strains to be able to survive. They are produced by many bacterial strains that can degrade or transform the components of petroleum products. They are non-toxic, non-hazardous, biodegradable and environmentally friendly compounds[5]
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