Development of theoretical model expressing self-beating deformation behaviour of human iPS cardiomyocytes tube

Abstract

In recent years, a regenerative therapy method using human iPS cell-derived myocardial cells (hiPS-CM) has attracted attention. In the future, large-scale myocardial three-dimensional structures that apply tissue engineering technology are expected. In this study, we developed a three-dimensional structure with a pump function by the self-beating power of hiPS-CM. Then, the deformation behavior of the 3D hiPS-CM structure associated with self-pulsation was examined. The pulsatile behavior of hiPS-CM was quantitatively evaluated by using a high-speed optical measurement system with the digital image correlation method. Then, the experimental strain data was used to construct the stress-strain response with use of a theoretical active stress model originally developed by Guccione et al. A theoretical constitutive model was also developed by combining the Guccione's active stress model and the viscoelastic Maxwell model. As a result, this theoretical model stress-strain behavior showed a good agreement with the combination model of experimental strain and theoretical stress.