Abstract
ABSTRACTThree models of a simple and inexpensive home-made gene gun apparatus was designed for genetic transformation of barley, wheat, maize and rice mature embryos. The compact and efficient design for gene transfer in plants was improved. The biological, physical parameters influencing transformation efficiency and bombardment medium suitable for gene guns apparatus were optimized. High levels of transient expression of the β-glucu-ronidase gene in the mature embryos of cereals were achieved.
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