Abstract
Nowadays, combined medicines are becoming more and more widespread. The combination of active pharmaceutical ingredients is necessary to increase the therapeutic effect or shorten the treatment period, or prevent possible complications. The aim of the work is to develop a method for the quantitative determination of biologically active compounds of dry extract of Bupleurum aureum as part of a combined dosage form in the form of a syrup in a mixture with loratadine.Methods. Identification of flavonoids in the extract was carried out by HPLC. To determine the quantitative content of flavonoid substances, the method of absorption spectrophotometry in the visible spectral range was used, based on the formation of colored complexes of flavonoids with a solution of aluminum chloride in an acidic medium.Results of the research. As a result of the research, a spectrophotometric method was developed to quantify the amount of flavonoids in the combined syrup with loratadine and Bupleurum aureum dry extract. The HPLC method establishes the flavonoids contained in the extract.The resulting colored complexes of alcoholic extracts from the syrup after the reaction of interaction with a solution of aluminum chloride in an acetic acid medium were characterized by the presence of absorption maxima at a wavelength of 412 nm. The effect of background absorption is insignificant noise δ = 0.25 %, max δ = 0.51 %. The studied validation characteristics of the technique, which indicate a linear dependence of the amount of flavonoids in terms of rutin in the range of concentration of Bupleurum aureum extract in syrup from 80 % to 120 %, since the value of the correlation coefficient (r) is 0.9999 ³ 0.9981; the angular coefficient of the linear dependence (b) is 0.9947, the free term of the linear dependence (a) is 0.52 £ 1.60. The technique is precise, since the value of the relative confidence interval is less than the critical value for the convergence of the results: D % = 0.37 ≤ 2.60 and the criterion of insignificance of systematic error is fulfilled d = 0.01.Conclusions. The HPLC method established the presence in the dry extract of the aerial part of the Bupleurum aureum substance of the flavonoid structure, which became a prerequisite for the standardization of the active substance in the syrup in the sum of these biologically active compounds. A spectrophotometric method of quantitative determination in the visible region of the sum of flavonoids in terms of rutin in the combined dosage form in the form of a syrup in the presence of another active ingredient loratadine has been developed
Highlights
Scientists call allergies, due to their excessive prevalence, as the epidemic of the XXI century
For the treatment of allergic manifestations, plant-based products are used, which can be conditioned by an increase in the interest of the population in preparations based on plant material and the spread of herbal medicinal products among the population
A new dosage form has been developed in the form of syrup, which, as active substances, includes the H1-histamine and serotonin receptor blocker loratadine and the dry extracts of the above-ground part of the Bupleurum aureum
Summary
Scientists call allergies, due to their excessive prevalence, as the epidemic of the XXI century. 5. Formulation of goals (tasks) of Article The aim of the work was to develop a method for quantitative determination of the biologically active compounds of the dry extract of Bupleurum aureum as a part of the combined dosage form in the form of a syrup in the presence of another active pharmaceutical ingredient loratadine and auxiliary substances. Quantitative content of loratadine in the combined dosage form was determined by spectrophotometric method in a 0.1 M hydrochloric acid solution at a wavelength of 278 nm after the previous removal of other active and auxiliary substances of the syrup by an organic layer [9, 10]. To determine the effect on the nature of the absorption spectrum of the resulting complex, a reaction with a solution of aluminum chloride, a combined syrup, a solution of extract in syrup (without loratadine) and placebo (Fig. 2) was performed
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