Development of the photosystem II unit in plastids of bean leaves greened in periodic light

Abstract

The photosystem II (PS II) unit formation and development, as monitored by the kinetics of the fluorescence induction, was studied in greening protochloroplasts isolated from etiolated bean leaves exposed to periodic light-dark cycles (LDC). It was found that: (i) The protochloroplasts show the well-known biphasic induction. The F MAX F O ratio increases with increasing exposure to LDC, and values almost twice as high as those of mature chloroplasts are reached. The fluorescence yield increases still more by the addition of NH 2OH. (ii) The ratio ( F MAX -F O ) F MAX , representing the yield of primary photochemistry, reaches values much higher than those of mature chloroplasts. (iii) The rate of fluorescence rise, in the presence or absence of 3-(3,4-dichlorophenyl)-1,1-di-methylurea (DCMU), is at least seven times slower than that of mature chloroplasts, and it remains constant during exposure to LDC. (iv) The shape of the fluorescence kinetics is exponential early during exposure to LDC but later it becomes sigmoidal, indicating the development of energy transfer between PS II units, (v) Dark incubation after a number of LDC increases the F MAX F O ratio without changing the rate of the fluorescence rise, (vi) Transfer of the plants from LDC to continuous illumination induces a decrease in the F MAX F O ratio and an increase in the rate of the fluorescence rise. The results indicate that initially small PS II units are formed, which contain mainly the reaction center with a few chlorophyll a molecules closely packed around it. At the same time H 2O-splitting enzymes are synthesized which, however, are light activated. These small units are very efficient for photochemistry. As the number of small units increases, aggregates are formed, which seem to have the reaction centers very close to each other. The aggregation of the units is controlled by the structural development and organization of the membrane and not by the concentration and type of chlorophyll. The excess chlorophyll formed after further exposure to continuous illumination is inserted into preexisting units, thus increasing their size and making them more efficient in absorbing the incident light.