Abstract
The aim of the study was to develop an optimal vitrification technology for the preservation and improve the survival of bovine and porcine cumulus-oocyte complexes (COCs). The objective of this study was to compare the efficacy of the vitrification of bovine COCs and porcine COCs by analysis of morphology after warming. Freezing of oocytes was carried out by vitrification in medium with different concentrations of various cryoprotectants (DMSO, EG and sucrose). The oocytes were transferred to a cryotube, which was transferred to liquid nitrogen. The warming procedure of oocytes was carried out in a solution of sucrose after 3-14 days of storage. Morphological assessment of oocytes and fluorescence microscopy were performed after the warming procedure. Survival of porcine COCs — 67.74%, bovine COCs — 81.25%. It was found that to assess the viability of oocytes should use fluorescent dye CAM. These results indicate that the our vitrification method without direct LN2 contact is more effective for vitrification of bovine oocytes.
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