Abstract
The article presents the method for the quantitative determination of flavonoids for the analysis of Bupleurum multinerve herb based on the method of differential spectrophotometry. The optimal conditions for analysis have been determined. They include extractant 40% ethyl alcohol, ratio of raw materials and extractant 1 : 100, extraction time of 60 minutes in the boiling water bath, complexing agent of 1 ml of 2% aluminum chloride. The use of rutin as a standard has been experimentally validated, analytical wavelength is 412 nm. The relative error of the mean result (for n = 9) was 3.20%. Validation studies of the method have shown that it meets the criteria: linearity (r = 0.99988), correctness, specificity, and precision. The analytical range of the method is 8.6726.08 g/ml. The method is recommended for the inclusion into the new edition of the Pharmacopoeia Monograph for this type of plant material.
Highlights
■ Предложена методика количественного определения флавоноидов для анализа травы володушки многожилковой (Bupleurum multinerve) на основе метода дифференциальной спектрофотометрии
■ The article presents the method for the quantitative determination of flavonoids for the analysis of Bupleurum multinerve herb based on the method of differential spectrophotometry
Стабилизация комплекса наблюдается процентного содержания проводят по рутину через 45 мин и сохраняется до 1,5 ч
Summary
Исследования проведены на образцах сырья B. multinerve, собранных в период массового цветения в южных районах Прибайкалья в 2018–2019 гг. Надземные органы B. multinerve срезали и сушили воздушно-теневым способом. Спектральные исследования проводили на спектрофотометрах LEKI SS 1207 и СФ‐2000 в кварцевых кюветах (1 см). Статистический анализ проводили при доверительной вероятности p = 95 % по ГФ XIV [2]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
