Development of the embryonic trisomy 1 mouse

Abstract

Trisomic and normal control embryos 9.5 through 12 gestational days were examined externally and on serial section with standard light microscopy techniques. Trisomic embryos showed significant retardation in length, weight, optic angle, and somite numbers at most times (p less than .05), and were 1/2 day behind normal animals in overall development. The eye showed the most defects, and these appeared at every period. They included aphakia, large intraretinal space, and dysmorphia of optic cup layers. Brain defects were found at all times. The most common were narrow brain vesicles and folding of the vesicle walls. Some animals showed neural tube defects. The major defect in neural crest derivatives was deficient frontonasal processes in the most defective embryos. The study supports the conclusion of previous investigators that Ts 1 is a syndrome of growth and developmental retardation with a wide range of defects. Definitive evidence, previously lacking, indicates that developmental delay and defective morphogenesis are apparent before 11 days of gestation. In addition, according to our measurements, optic angles are larger in trisomic mice than in normal littermates. This is in contrast to the only previous investigation that included such measurements. Our data do not give definitive support to the suggestion that trisomy 1 belongs to the holoprosencephaly-cyclopia spectrum. However, the hypothesis that murine trisomy 1 shows a parallel in development to human trisomy 13 is not supported by our data. Possible causal mechanisms for the defects and developmental delay are discussed.