Abstract
In this study, we developed a novel DNA sensor exploiting Carbon/La3+/CuO (C/La3+/CuO) nanocomposites for the determination of isoniazid (INZ). After modification of sensor by C/La3+/CuO, the electrode showed an excellent conductivity, and carbon possesses a large specific surface area, thereby enabling a significant improvement in the sensor sensitivity. Since no DNA probe modification or chemical labeling was required in this approach, this sensor could be employed to detect INZ. The present biosensor was selectively and sensitively able to detect INZ, with a wide linear range (1.0–165.0 μM) and a low limit of detection (0.035 μM). The fabricated biosensor was also used to detect INZ in the real samples successfully. Good recovery values were obtained, showing the excellent analytical potential of the electrochemical biosensor based on ds-DNA/C/La3+/CuO. Moreover, electrochemical, spectroscopic and docking methods showed an effective interaction between INZ and ds-DNA.
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