Abstract
Dorsal vessels of Schistocerca americana were maintained for 6 days in organ culture systems with a medium consisting of 60% M-18, 30% modified Grace's medium, and 10% fetal bovine serum. Homogenates of the dorsal vessels that were exposed to medium containing the crystalline-array virus (CAV) showed increased virus activity compared with the original inoculating medium at 6 days after exposure, and most adsorption occurred during the first hour of exposure. Further testing indicated that a major portion of increased virus activity occurred within 24 hr after infection which suggested that the viral particles were incorporated into crystals which were not dispersed when injected into the hemocoel of grasshoppers.
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