Abstract
BackgroundAcetoin is a vital platform compound widely used in the food, pharmaceutical, and chemical industries. Since 2004, the United States Department of Energy has listed it as one of the 30 platform compounds for priority development. Bacillus subtilis, a generally recognized safe microorganism, is an ideal platform microorganism for the engineered production of valuable bio-based chemicals. MethodsThe Cre-Cas 2.0 system derived from the Cre-Cas system was applied to inactive, enhance, or recover native metabolic genes. This system was designed to evaluate which native metabolic genes affect acetoin production in B. subtilis. Significant findingsUtilizing the Cre-Cas 2.0 system, acetoin reductase (bdhA), l-lactate dehydrogenase (ldh), and acetoin dehydrogenase (acoA) were inactivated, while the native acetoin synthesis genes, alsSD operon and alsR, were enhanced. This approach resulted in the creation of sixteen recombinant strains for the assessment of acetoin production. Finally, the acetoin production of strain ACN11 was 55 % higher than the original strain B. subtilis DB428. Moreover, employing the Cre-Cas 2.0 concept for editing the chromosomal genes of B. subtilis to enhance acetoin and other biochemical production could offer flexibility and applicability.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
