Abstract
We developed a non-invasive DNA sampling method and 15 tetranucleotide microsatellite markers for Texas horned lizards (Phrynosoma cornutum). Swabbing the cloaca with a small cotton swab and preserving the cells in lysis buffer was an effective method to obtain tissue for DNA extraction. Loci were highly polymorphic with 8–25 alleles and observed heterozygosity was high (0.71–0.96). Some of these loci can also be used for round-tailed horned lizards (P. modestum).
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