Development of supercritical fluid extraction with a solid-phase trapping for fast estimation of toxic load of polychlorinated dibenzo- p-dioxins-dibenzofurans in sawmill soil

Abstract

A method consisting of automated supercritical fluid extraction (SFE) with simultaneous cleanup by a solid-phase trap was developed for fast analysis of polychlorinated dibenzo- p-dioxins (PCDDs) and dibenzofurans (PCDFs) in soil. SFE was optimised to replace conventional liquid-based methods in routine analyses of PCDD/PCDFs in sawmill soil contaminated by a chlorophenol formulation. PCDD/PCDFs were quantitatively extracted in 60 min using CO 2 at 400 atm and 100 °C without a modifier. A trap containing a small amount of activated carbon mixed with Celite efficiently collected PCDD/PCDFs after SFE. After SFE co-extracted impurities were eluted out from the trap with 4 ml of hexane and PCDD/PCDFs were eluted with 10 ml of toluene. The concentrations and TCDD-equivalent of PCDD/PCDFs corresponded to the results of traditional solvent extraction method (Soxhlet) in six sawmill soils tested. The performance of the trap was maintained over a long period of time (nearly 100 extractions).