Abstract
A simple, sensitive an isocratic RP-HPLC method for the estimation of Metformin Hydrochloride (MET) and Glipizide (GPZ) in combined dosage form using Inertsil C8 column (250×4.6 mm, 5 µ) in an isocratic mode with mobile phase comprising Acetonitrile: Water (70:30) & one drop of Triethylamine. The flow rate was 1.0 ml/min and effluent was monitored at 222 nm. The retention times were found to be 3.40 min for MET and 4.44 min for GPZ. The assay exhibited a linear dynamic range of 100-500 µg/ml of MET and 1-5 µg/mL for GPZ .The calibration curves were linear (r = 0.9985 for MET and r = 0.9955 for GPZ) over the entire linear range. Recovery was found to be 99.94 % + .17 for MET and 99.61 % + 0.89 for GPZ. % RSD of system precision were observed 0.0429 for MET & 0.7212 for GPZ.
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