Abstract

To develop a simple and convenient marker for genetic linkage of sesame male sterile gene,based on an AFLP marker,7 SCAR( sequence characterized amplified region) markers were developed using recessive genic male sterile two- type line 95ms- 5AB as fertile and sterile gene bulks. These 7 markers could detect bands from fertile bulk with 806 bp to 1 638 bp in size,and could not from sterile bulk. They were designated as PMC- 1to PMC- 7. These markers were used to detect 1 184 fertile and sterile plants of 95ms- 5AB with 0. 25% exchange rate. 4 F2 populations obtained from 95ms- 5A and different genotypes and 2 recessive genic male sterile lines were identified using these 7 SCAR markers. Result indicated that the SCAR markers were specific for 95ms- 5.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call