Abstract
Cinnamomum verum (true cinnamon) and Cinnamomum cassia (cassia cinnamon) are two important species belonging to family Lauraceae. These species are recognized by morphological, chemical composition and essential oil contents. The appropriate identification of species would be considerably improved by a genetic method. The main objective of the present study was to develop molecular markers distinguishing between C. verum and C. cassia. A total 71 ISSR (Inter simple sequence repeat) and four universal barcoding (ITS, rbcL, matK,andpsbA-trnH) genes were used to distinguish both the species. No sequence variation was observed between the two species for any DNA barcode gene. However, one ISSR i.e. ISSR-37 showed a clear distinction between the species and produced 570bp and 746bp amplicons in C. verum and C. cassia, respectively. The polymorphic bands were converted into species-specific SCAR markers. The SCAR-CV was specific to C. verum and amplified 190bp band, however there was no amplification seen in the C. cassia samples. The SCAR marker generated in this study can be employed as efficient, economical, and reliable molecular tool for the identification of C. verum.
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