Abstract
Protein imprinted MIPs show notable potential for applications in many analytical areas such as clinical analysis, medical diagnostics and environmental monitoring, but also in drug delivery scenarios. In this study, we present various modifications of two different synthesis routes to create imprinted core-shell particles serving as a synthetic recognition material for the protein hen egg white (HEW) lysozyme. HEW lysozyme is used as food additive E 1105 for preservation due to its antibacterial effects. For facilitating quality and regulatory control analysis in food matrices, it is necessary to apply suitable isolation methods as potentially provided by molecularly imprinted materials. The highest binding capacity achieved herein was 58.82 mg/g with imprinting factors ranging up to 2.74, rendering these materials exceptionally suitable for selectively isolating HEW lysozyme.
Highlights
We present various modifications of two different synthesis routes to create imprinted core-shell particles serving as a synthetic recognition material for the protein hen egg white (HEW) lysozyme
HEW lysozyme is used as food additive E 1105 for preservation due to its antibacterial effects
Molecular imprinted polymers (MIPs) show notable potential for applications in many analytical areas, such as clinical analysis, medical diagnostics, environmental monitoring, but more recently for assisting drug delivery [4,7]. They can be employed in high-performance liquid chromatography [8], solid phase extraction [9], sensors [10], separation [11,12], and catalysis [13,14]
Summary
The technology of molecular imprinting enables the creation of artificial binding sites within a polymer matrix These sites are tailored in situ via co-polymerization of functional monomers and crosslinkers around the template [5,6]. Afterwards, the template molecule is extracted from the obtained polymer, leaving complementary cavities (regarding shape, size, and distribution of functional groups) within the polymeric network [1,2,3,4] For this reason, MIPs show notable potential for applications in many analytical areas, such as clinical analysis, medical diagnostics, environmental monitoring, but more recently for assisting drug delivery [4,7]. Small molecules are easier to imprint than macromolecules or entire biological species (i.e., peptides, proteins, DNA, viruses and bacteria), which can be limited by size, mass transfer, conformational instability, etc. [15] mass transfer to and through the crosslinked polymer matrix is
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