Abstract
Portulaca quadrifida (Portulacaceae) is an annual succulent herb having medicinal value and is consumed as a vegetable or salads in India. In the present study, leaf explants were inoculated on Murashige and Skoog’s (MS) medium fortified with sucrose (3%) and combinations of N6-benzyladenine (6-BA) and kinetin (KIN) individually and in combination with 1-naphtalene acetic acid (NAA). Rapid regeneration was observed in medium fortified with combinations of 6-BA (8 µM) and NAA (1 µM) which formed 19.40 ± 0.64 shoots with 100% response. Variation in sucrose concentrations (4-6%) was tried but it failed to increase the shoot number. When the optimized medium was fortified with different carbon sources viz. dextrose, glucose and maltose, they could not evoked better response and sucrose proved to be more effective for regeneration. Rooting of in vitro shoots was achieved in ½MS + sucrose (1%) + indole-3-butyric acid (IBA, 2 µM).
Highlights
Portulaca quadrifida L. commonly called ‘looni’ which belongs to the family Portulacaceae is a small diffused, succulent, annual herb found throughout the tropical parts of India
Culture medium for shoot regeneration Murashige and Skoog (MS, 1962), medium fortified with sucrose (SRL, Mumbai, India) (3%) served as a control for the study
Establishment of shoot cultures from leaf explant Entire leaves of P. quadrifida when inoculated on basal MS medium as well as on medium with 6-BA and KIN individually, resulted in only swelling and failed to induce any morphogenic response
Summary
Portulaca quadrifida L. commonly called ‘looni’ which belongs to the family Portulacaceae is a small diffused, succulent, annual herb found throughout the tropical parts of India. The plant has medicinal properties and is used to cure asthma, cough, urinary discharge, inflammation, ulcers, abdominal complaints, erysipelas and hemorrhoids (Kirthikar and Basu, 2001; Mulla and Swamy, 2010). It has neuropharmacological effects and antifungal activities against Aspergillus fumigates and Candida albicans (Hoffman et al, 2004; Syed et al, 2010). Due to its nutritional and medicinal importance it was taken up for regeneration studies
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