Abstract
Pointed gourd ( Trichosanthes dioica Roxb .), a perennial herb of family Cucurbitaceae, is morphologically different from the other cucurbits due to its dioecism and vegetative means of propagation. Breeding in pointed gourd is less viable due to poor germination, vegetative propagation mean and dioecy. A reliable method for determining the gender of pointed gourd at seedling stage would facilitate breeding programs. With this aim, RAPD markers were used to identify a sex linked marker. RAPD profiles with 104 primers were analyzed in 10 male and 44 female plants for presence/absence of specific bands. Two sex-specific fragments, OPC05 1000 and OPK07 300 , associated with maleness and femaleness, respectively, were converted in SCAR markers. Based on the sequence of OPC5 1000 and OPK-07 300 , five and one pair of specific forward and reverse SCAR markers were designed, respectively. Among five male specific SCAR, only one marker (SCF/R-3 628 ) discriminated both sexes. Thus, SCAR designed from RAPD fragment could be used as an effective and reliable marker for early sex diagnosis and to reduce both time and costs associated with breeding in T. dioeca .
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
