Abstract
The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2 The kits included enzyme reaction mixtures and chromogenic agents After the isothermal amplification reactions were completed, the reaction results were judged by using the chromogenic agents to determine whether SARS-CoV-2 exists in the samples to be tested The detection kits have the advantages of convenient operation, fast detection speed and high sensitivity up to 1 copy of virus particles per reaction, which can speed up the detection speed of suspected cases, and avoid the missing detection problems caused by the low detection sensitivity
Highlights
From December 2019, COVID-19 caused by SARSCoV-2 (2019-nCoV) has become a public health event of global concern [1,2,3]
The isothermal amplification reactions are performed at 63 °C for 40 minutes and the enzyme was inactivated at 82 °C for 7 minutes
The SARS-CoV-2 nucleic acid detection kits based on colorimetric loop-mediated isothermal amplification (LAMP) technology were provided for clinical diagnosis, which is of great significance to the surveillance and detection of COVID-19 pandemic
Summary
From December 2019, COVID-19 caused by SARSCoV-2 (2019-nCoV) has become a public health event of global concern [1,2,3]. 50× of the final concentration of Sybr Green I, GeneFinder and GelRed were added to the amplification products respectively, to determine the sensitivity of the detection kits with the naked eyes, or the ultraviolet light at 254 nm of wavelength.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
