Development of rice mutant tolerance to salinity through combination of gamma rays irradiation and in-vitro selection

Abstract

Global warming caused a large rise in global sea level poses many threats, especially to a country like Indonesia. One of the threats that affected rice field located adjacent to coastal area is increase level of soil salinity. Inpari 34 and Inpari 35 varieties tolerant to salinity at seedling phase cannot perform well when planted in salinity prone area where the increase in salinity affected by the up and down of sea surface occurred during the planting season. The aims of this experiment were to obtain putative salinity-tolerant mutant (M1) at vegetative and generative phase from Inpari 34 and Inpari 35 through combination of gamma rays irradiation and in-vitro selection. The study consisted of several activities, namely embryogenic callus induction, mutation induction by gamma-ray irradiation, callus in-vitro selection by NaCl, regeneration of selected callus, and plantlet acclimatization and greenhouse grow-out. In this experiment 62 and 54 putative salinity-tolerant mutant lines obtained from Inpari 34 and Inpari 35, respectively.