Abstract
BackgroundThe emerged human infection with avian influenza A (H7N9) virus in China since 2013 has aroused global concerns. There is great demand for simple and rapid diagnostic method for early detection of H7N9 to provide timely treatment and disease control. The aim of the current study was to develop a rapid, accurate and feasible reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of H7N9 virus.ResultsThe detection limits of the H7- and N9-specific RT-LAMP assay were both approximately 0.2 PFU per reaction. No cross-reactivity was observed with other subtype of influenza viruses or common respiratory viral pathogens. The assay worked well with clinical specimens from patients and chickens, and exhibited high specificity and sensitivity.ConclusionsThe H7/N9 specific RT-LAMP assay was sensitive and accurate, which could be a useful alternative in clinical diagnostics of influenza A (H7N9) virus, especially in the hospitals and laboratories without sophisticated diagnostic systems.
Highlights
The emerged human infection with avian influenza A (H7N9) virus in China since 2013 has aroused global concerns
The results demonstrated that the reverse-transcription loop-mediated isothermal amplification (RT-loop-mediated isothermal amplification (LAMP)) assay was sensitive and accurate, which could be a useful alternative in clinical diagnostics of influenza A (H7N9) virus, especially in the hospitals and laboratories without sophisticated diagnostic systems
The detection limits of the H7 and N9 specific RTLAMP assay were both 0.2 Plaque Forming Unit (PFU) per reaction (Figure 1A and C), which was 10-fold-higher sensitive than that of real-time Reverse transcription polymerase chain reaction (RT-PCR) assay recommended by World Health Organizatin (WHO) (Table 2)
Summary
The emerged human infection with avian influenza A (H7N9) virus in China since 2013 has aroused global concerns. There is great demand for simple and rapid diagnostic method for early detection of H7N9 to provide timely treatment and disease control. The aim of the current study was to develop a rapid, accurate and feasible reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of H7N9 virus. Since February 2013,a novel avian influenza A (H7N9) virus has emerged in China, resulting in human infections [1]. Human infection with avian influenza A (H7N9) virus usually results in an influenzalike illness (ILI) with symptoms such as fever, cough with little to no sputum production, accompanied by headache, muscular soreness, and general malaise [6]. Considering that the novel avian H7N9 is characteristic of mammalian adapted, scientific community
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