Abstract
Developing reusable, sustainable and long term storable bioremoval technologies is essential for removal of heavy metals and reactive dyes in industrial wastewater. <i>Clavibacter michiganensis</i> (<i>C. michiganensis</i>) having bioremediation property was selected for attachment, and polysulfone (PSU) was selected as the polymer matrices for the electrospinning of nanofibrous webs. For this approach, polysulfone nanofiber mats (PSU-NFMs) were produced by electrospinning, followed by attachment of bacteria onto nanofiber mats and a subsequent used in the removal of Cr(VI) and reactive dye. The efficient Cr(VI) removal with 100% yield was achieved within 24 hours (10 ppm) with bacteria attached nanofiber mats. Also, nano bio-composite mats decolorized 100% of Reactive Blue (RB, 20 ppm) within 13 h. Furthermore, the prepared bacteria attached to PSU-NFMs showed storage stability at 23 ± 2℃ and 4℃ for 30 days with efficient removal yields and PSU-NFMs found as potentially storable for keeping attached bacterial cells alive. Finally, the reusability of bacteria attached to PSU-NFMs were determined after five cycles for both pollutant and 70.3% and 51.8% of the Cr(VI) and dye removal capacities were obtained. It was expected that the bacteria attached to PSU-NFMs could be a potential application in wastewater treatment.
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