Abstract
Introduction: Most Philadelphia-positive (Ph+) Acute Lymphoblastic Leukemia (ALL) alleles are BCR1ABL1 p190 fusion transcripts. Objectives: Enhance and test acute lymphoblastic leukaemia BCR-ABL1 p190 transcript finding and tracking RT qPCR tool. Methodology: Cycle frequency, primer concentration, and real-time qPCR output, volume, and results improve acute lymphoblastic leukaemia data. Normal curve and element analysis needed enough cDNA. Before cDNA, Nanodrop tested distal blood RNA. Nanodrop RNA measures and extracts RNA. We used specialised gear and BCR-ABL P190 primers to find a genomic variety. Numerous samples were examined for accuracy. Trials lasted 1–3 days. Results: 15 BCR-ABL+ALL tissues had CT values around 25 cycles. The water stopped errors. Retested parts. 45 chronic myeloid and 15 acute lymphoblastic leukaemia samples were BCR-ABLp190-negative using the same method. Conclusion: Real-time PCR found BCR-ABL1. Better BCR-ABL1 p190 detection and lab accuracy allow real-time qPCR to track ALL.
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