Abstract
African swine fever virus (ASFV) can infect domestic pigs and wild boars and causes huge economic losses in global swine industry. Therefore, early diagnosis of ASFV is important for the control and eradication of African swine fever (ASF). In this study, a SYBR Green-based real-time polymerase chain reaction (PCR) assay targeting the viral encoded A137R gene was established for the detection of ASFV infection. For the evaluation of the established real-time PCR, 34 clinical samples were assessed by both the A137R gene-based real-time PCR and OIE-recommended TaqMan PCR. The results showed that 85.29% (29/34) were detected by A137R gene-based real-time PCR, but only 79.41% (27/34) positive using OIE-recommended TaqMan PCR. Moreover, no cross-reaction with other common swine pathogens was found in the A137R gene-based real-time PCR. These results demonstrated that the established real-time PCR assay in this study showed better performance than the OIE-recommended method in detecting ASFV from clinical samples, which could be applied for control and eradication programs of ASF.
Highlights
African swine fever (ASF) is a contagious viral disease characterized by fever, cutaneous congestion, multiple hemorrhages in the internal organs, ataxia, severe depression, and high mortality in acute cases
The detection limit is given as the lowest detectable dilution of the DNA
Selected DNA of African swine fever virus (ASFV) positive specimens in Table 3 showed that A137R gene-based real-time PCR had an equal or 10 times higher sensitivity when compared with the OIE-recommended TaqMan PCR
Summary
African swine fever (ASF) is a contagious viral disease characterized by fever, cutaneous congestion, multiple hemorrhages in the internal organs, ataxia, severe depression, and high mortality in acute cases. ASF spreads to the entire Southeast Asia in the year [2,3,4]. The outbreaks of ASF are still ongoing in Africa, the trans-Caucasus region, Eastern Europe, Russian Federation and Asia, which caused a huge challenge to the swine industry in these regions [4]. The improvement of African swine fever virus (ASFV) diagnostic methods is one of the essential steps to control and prevent the ASF. Considering the similarities in clinical symptoms between ASF and other swine diseases, such as classical swine fever (CSF), and the potential appearance of non-specific clinical symptoms, the rapid and reliable diagnosis is essential for timely
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
