Abstract
Propolis as natural resources from bee has complex biological and chemical depending the plant around the hives. This study aimed to find out cytotoxic activity of n-hexane fractions of propolis from Madu Efi’s bee farm N-hexane and the maceration method were used to make the fraction. Using the MTT method on WiDr cell lines for 24 and 48 hours, cytotoxic activity was measured. The MTT test showed that the IC50 values for the n-hexane fraction and 5-Fu were 74.16 0.11 g/mL; 54.40 0.78 g/mL for 24 h; and 49.65 1.03 g/mL; 33.86 0.58 g/mL for 48 h. The results show that WiDr cell lines are killed by n-hexane fractions of propolis from MaduEfi’s bee farm. The best time to incubate them is for 48 hours.
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