Development of Probiotic Lactobacillus sporogenes Loaded Biological Macromolecules using Novel Assembly Design for Long-term Preservation as Bio-therapeutic Agent

Abstract

The goal present investigation was to formulate and characterized of biological macromolecules of alginate (ALG) and carboxymethylcellulose sodium (CMC) containing probiotic bacteria of Lactobacillus sporogenes (LS) co-encapsulated with a prebiotics, Bioecolians (α-Gluco-oligosaccharides). The prepared beads were characterized in terms of yield, size, encapsulation efficiency, viabilities in simulated gastric (pH 1.2, 2 hours) and bile (1% w/v, 3 hours) conditions. The beads were also characterized by FTIR, DSC, SEM and XRD to investigate molecular structure, surface properties and morphology of beads. The results showed that spherical beads with size distribution ranging from 1.18 ± 0.11 to 1.45 ± 0.15 mm for ALG and from 1.3 ± 0.12 to 1.5 ± 0.16 mm for ALG-CMC with encapsulation efficiency higher than 90% were achieved. The results indicated that incorporation of carboxymethylcellulose sodium into alginate beads improved viability of the bacteria in simulated gastric conditions as well as bile conditions. According to our in vitro studies, Probiotic beads using combination of ALG-CMC are suitable encapsulating polymer for gastro-intestinal delivery as designed by novel assembly using peristaltic pump for automated production.