Abstract
Cycloamylose (CA) can entrap molecules to form inclusion complexes. Here, we report the potential application of enzymatically produced CA from low-cost tapioca starch as a nanocarrier for human papillomavirus type 16 (HPV16) E6 siRNA in cervical cancer treatment. The cationic charged CA (CAQ) was produced by the introduction of (3-Chloro-2 hydroxypropyl) trimethylammonium chloride (Q188) to the particle, resulting in successful complexation of CAQ and siRNA. This was then transfected into the cervical cancer cell line (CaSki). The CAQ-siRNA complex demonstrated no cytotoxicity in normal cells and exhibited the same level of transfection efficiency compared to a commercial liposome-based particle. In contrast, the commercial liposome-based particles showed a 40 % decrease in the viability of normal cells. These results suggest that low-cost, high biocompatibility and easy-to-use CAQ is a feasible alternative for an siRNA carrier. This new system had no impact on cell viability and has the potential to be used in medical applications.
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