Abstract
Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification
Highlights
Portable nucleic acid test systems for on-site diagnosis have attracted a lot of attention.[1,2,3] Carrying out nucleic acid testing in the field is useful for point-of-care testing,(4)environmental analysis,(5) and food safety testing[6] because the result can be obtained rapidly.To miniaturize and reduce the cost of nucleic acid test systems, the design of the detection and heating parts is very important
Loop-mediated isothermal amplification (LAMP),(11) which is one of the nucleic acid amplification methods, is very useful for on-site nucleic acid testing since it is capable of rapid nucleic acid amplification by isothermal heating
Some detection devices have been developed for real-time LAMP reaction monitoring, such as a small detection system using an LED and a photodiode (PD),(22,23) a tube scanner,(24) and a smartphone-based detection system.[25]. These detection devices were small and easy to use and had a high detection sensitivity and a low fabrication cost
Summary
Portable nucleic acid test systems for on-site diagnosis have attracted a lot of attention.[1,2,3] Carrying out nucleic acid testing in the field is useful for point-of-care testing,(4)environmental analysis,(5) and food safety testing[6] because the result can be obtained rapidly.To miniaturize and reduce the cost of nucleic acid test systems, the design of the detection and heating parts is very important. LAMP can be adapted to various detection methods [e.g., turbidimetry[12] and colorimetry[13]] as well as to the fluorescence detection method,(14,15) and LAMP enables us to discriminate the presence or absence of gene amplification visually.[16,17,18] isothermal heating enables us to simplify the system configuration Owing to these advantages, many research groups have studied LAMP systems based on visual detection for on-site nucleic acid testing.[19,20,21] visual discrimination is simple and does not require a detection device, the obtained result is semi-quantitative. Real-time signal monitoring during the LAMP reaction[22] is useful for rapidly finding abnormalities caused by contamination as well as for the quantitation of nucleic acid. The performance of these heaters is sufficient to perform the LAMP reaction, but the optical arrangement of the detection system is limited because of the opaque heater material
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