Abstract
A plaque assay method, using 1.05% methyl cellulose overlay, was developed for vesicular exanthema of swine virus (VESV) types A48, H54, I55, and K54. The validity of this plaque method as an assay system was upheld by the well correlated dose response, and the specificity of plaque formation was demonstrated by the fact that a specific antiserum could reduce the plaque count by more than 98%. The concentration of methyl cellulose exerted an influence on the size of plaques formed by the virus, showing an inverse relationship between concentration of methyl cellulose in the overlay medium and plaque size. Adsorption of VESV to monolayers of pig kidney cells at 37° C was found to reach a maximum within 60 minutes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
