Abstract
Thiocyanate peroxidase (TPO) activity of the submandibular gland of mice began to appear on the 17th day in utero and increased with time. This enzyme activity was lacking in the sublingual gland. Cultured glands of embryonic mice in a Rose chamber revealed proliferation and differentiation in both submandibular and sublingual glands. After 3 days cultivation, peroxidase was detected in the cells of the terminal tubules of the submandibular gland, but not in the sublingual. TPO activity measured by 14CO 2 production from [ 14C]-thiocyanate by cultured composite submandibular and sublingual glands became evident on the 4th day of cultivation. The results suggest that the peroxidase activity involved in the TPO system is of salivary gland origin.
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