Abstract
Event Abstract Back to Event Development of orally available bile acid conjugated nanoparticle using heparin and protamine Jooho Park1 and Youngro Byun1, 2 1 Seoul National University, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Korea 2 Seoul National University, Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Scie, Korea Oral delivery is the most clinically acceptable delivery route for patients. However, it is hard to deliver some therapeutic agents including macromolecule, peptide and gene. For decades, several researchers have tried to develop an oral agent for macromolecule delivery [1],[2]. Especially, nanoparticles and nanocomplex are remarkable carriers for therapeutic agents, but, they are not usually available as an oral tablet [3]. Here, we developed a new nanoparticle using two FDA-approved biomaterials for oral delivery. A negatively charged heparin and positively charged peptide named protamine have shown clinical therapeutic potential [4]. Using the polysaccharide, peptide and bile acids, we have designed an orally available nanoparticle. It was possible to control the surface charge and the size of nanoparticle by using the formulation of heparin and protamine. Moreover, the bile acids in nanoparticle enhanced the oral delivery of the nanoparticle. This nanoparticle could bind to the apical sodium-dependent bile acid transporter (ASBT) on caco-2 and MDCK cell. In addition, the nanoparticles were not in the tight junctions but they were present in the transcellular region; chitosan-based positive nanoparticles are usually absorbed via paracellular route. Furthermore, cy5-conjugated nanoparticle was treated to rats and mice to detect the nanoparticles in the GI tract. The orally treated nanoparticles were absorbed in the ileum, and then the retention period of the nanoparticles was also measured by using cy5 signals. In conclusion, these results represent an advanced major oral delivery technique for drug and gene delivery. The oral delivery of safe and functional nanoparticles would invoke the benefits of advanced oral delivery system providing a clinical therapeutic potential. This study was supported by grants from the Bio & Medical Technology Development Program (grant no. 2012028833). The authors thank National Center for Inter-University Research Facilities for technical assistance.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.